The fermentation of galactose and galactose-1-phosphate

Simultaneous Quantitative Galactose-1-Phosphate and Blood for the Diagnosis of Estimation of Galactose in Galactosemia

FUJIMURA, Y., KAWAMURA, M. and NARUSE, H. Simultaneous Quantitative Estimation of Galactose-1-Phosphate and Galactovve in Blood for the Diagnosis of Galactosemia. Tohoku J. exp. Med., 1982, 137 (3), 28°-295 A new microfluorometrical simultaneous assay method of galactose-l-phosphate and galactose in blood discs was devised by use of alkaline phosphatase and fl-galactose dehydrogenase. Our metho...

Galactosemia screening by simultaneous blood spot quantification of galactose and galactose 1-phosphate.

The Philadelphia chromosome and galactose-1-phosphate uridyl transferase.

\ ‘7ith the technical assistance of Jean Downes T FIE OCCURRENCE OF chromosomal abnormalities in humans provides a method for investigating the function of specific chromosomes. In trisomy-21 many studies have l)een carried out in an attempt to evaluate the functions of this autosome.l h Another abnormality of the 21 group is found in patients with chronic myelogenous leukemia. In these cases, ...

Lactose and D-galactose metabolism in Staphylococcus aureus. II. Isomerization of D-galactose 6-phosphate to D-tagatose 6-phosphate by a specific D-galactose-6-phosphate isomerase.

The inducible D-galactose-6-phosphate isomerase that functions in the metabolism of lactose and D-galactose in Staphylococcus aureus was partially purified from extracts of D-galactose-grown cells. It was shown to catalyze specifically the reversible isomerization of D-galactose 6-phosphate to D-tagatose 6-phosphate, the apparent Km values being 9.6 mM and 1.9 mM, respectively. At equilibrium, ...

A mouse model of galactose-1-phosphate uridyl transferase deficiency.

Galactose-1-phosphate uridyl transferase (GALT) deficiency causes classical galactosemia in humans. Mice deficient in this enzyme were created by gene targeting. GALT-deficient mice develop biochemical features similar to those seen in humans with GALT deficiency, but fail to develop the pattern of acute toxicity seen in newborns with classical galactosemia. This study suggests that alternative...